Inflammation Biomarkers of Advanced Disease in Nongingival Tissues of Chronic Periodontitis Patients

Figure 1

Histopathological analysis and cytokine and collagen production in tissues extracted from control and periodontal disease (PD) patients. Samples were fixed, decalcified, embedded in paraffin, and stained with Hematoxylin and Eosin (HE) or tartrate-resistant acid phosphatase (TRAP) staining protocol. (a) and (b) represent the HE slides from bone and adjacent tissue of control and PD group at 200x, respectively. (c) depicts the number of TRAP positive cells in the bone and adjacent tissue of control ( and PD () subjects. Immunohistochemical staining is shown for TGF-β (d) and IL-17 (e) for control () and PD () subjects. In (f), the percentage of Picrosirius stained areas for collagen quantitation in control and PD group, as described in Section 2..

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