PGE2 Elevates IL-23 Production in Human Dendritic Cells via a cAMP Dependent Pathway

Figure 2

Influence of PGE2 on MyD88-mediated IL-23 p19 promoter activity of HEK293T cells. Different doses of MyD88 (0–40 ng) were cotransfected with IL-23 p19 (100 ng); then the IL-23 p19 promoter activity was assayed (a). In addition, the effect of PGE2 on IL-23 p19 promoter activity was detected (b). In brief, after being pretreated with PGE2 (0.1–8 μM) or DMSO for 1 h, HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 reagents. Luciferase units were divided by their renilla control, and the empty vector control was then subtracted. Fold change was calculated by dividing each experimental value by the value for the control group. Data are representative of 3 separate experiments. versus no treatment.

(a)

(b)