Mediators of Inflammation / 2016 / Article / Fig 6

Research Article

Resveratrol Protects against Sepsis-Associated Encephalopathy and Inhibits the NLRP3/IL-1β Axis in Microglia

Figure 6

In vitro assay of the effects of Sirt1 inhibitor, NAM, on the protective effects of resveratrol in BV2 cells. Blots portray protein expression of NLRP3, caspase-1, and IL-1β. All immunoblots were normalized to the loading control, β-actin. This population of BV2 cells was treated with resveratrol, either in the presence or in the absence of nicotinamide. Control cells were stimulated with ATP alone, while LPS cells were stimulated with both ATP and LPS. Res cells were treated with one dose of resveratrol at a concentration of 30 μM, in the presence of both ATP and LPS. NAM cells were treated with the same dose of resveratrol (30 μM) but in the presence of nicotinamide (10 mM), ATP, and LPS. compared with control group, compared with LPS group, and compared with Res group, by one-way ANOVA and LSD tests. Bars represent the mean ± SD.