Krüppel-Like Factor 4 Is a Regulator of Proinflammatory Signaling in Fibroblast-Like Synoviocytes through Increased IL-6 Expression

Figure 3

KLF4 overexpression induces IL-6 expression in RA FLSs. RA FLSs were transiently transfected with pcDNA3.1-KLF4 (KLF4) or the pcDNA3.1 empty vector (Neo) and then incubated with TNF-α (20 ng/mL). Expression of KLF4 was assessed after 3 h by real-time PCR (a) and after 12 h by western blotting (b). Expression of IL-6 was assessed after 3 h by real-time PCR (c) and ELISA (d). GAPDH as an endogenous control. Data were expressed as mean ± SEM (); in comparison to the untreated cells (Ctrl); in comparison to the TNF-α group.

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