Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFB

Figure 6

NFκB1 mRNA and IκBα protein levels in differentiated 3T3-L1 cells treated with LPS in the absence or presence of FK. Differentiated 3T3-L1 cells were treated as described in Materials and Methods under the following conditions: CTL, 10 µM FK, 1 µg/mL LPS, and LPS + FK. (a) The results are expressed as mRNA levels (fold stimulation over CTL set to 1). (b) Phospho-IκBα and total IκBα protein levels were determined in DC by Western blot analysis. (c) Semiquantitative determination of protein expression was performed as described under Materials and Methods. The results are expressed as protein expression (ratio of total IκBα or P-IκBα band density over β-actin band density) expressed as percent of the CTL value (CTL set to 100%) and are the mean ± SEM () (in % of CTL). Data were analyzed using -test for unique sample and paired -test; versus CTL; versus LPS.

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