Sphingosine-1-Phosphate/Sphingosine-1-Phosphate Receptor 2 Axis Can Promote Mouse and Human Primary Mast Cell Angiogenic Potential through Upregulation of Vascular Endothelial Growth Factor-A and Matrix Metalloproteinase-2

Figure 3

Effect of S1P on VEGF-A and MMP-2 mRNA expression. Human (a, b) and mouse (c, d, BMMC) MC were stimulated in the presence of S1P (100 nM) for different periods of time at which VEGF-A (a, c) and MMP-2 (b, d) mRNA levels were measured by QPCR. mRNA was prepared from 3 independent BMMC and 5 independent human MC populations, in duplicate. Kinetics of S1P stimulation was repeated 3 to 4 times; each QPCR experiment was performed in duplicate determination for each individual sample. When reaching significance, statistics are indicated in each figure. Error bars show standard error of means. .

(a) Skin mast cells

(b) Skin mast cells

(c) BMMC

(d) BMMC