FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids

Figure 2

Ffar4 HET and KO mice are protected against insulin resistance on a high fat ω3-PUFA diet. Insulin tolerance tests were performed at week 33 after diet initiation in 2-hour food-deprived mice. An insulin bolus of 1 U/kg lean mass was injected i.p. ((a)–(c)) Heterozygotes. ((d)–(f)) Knockouts. ((a) and (d)) ITT, –8; ((b) and (e)) 5-hour fasting insulin levels 32 weeks after diet initiation, –8. ((c) and (f)) Plasma adiponectin levels 36 weeks after diet initiation, –7. All data are presented as means ± SEM. Both HFDs have been compared to the LFD. For (a) and (d), 2-way RM ANOVA with Bonferroni correction was performed. All bar graph data were Ln-transformed and subjected to 1-way ANOVA with Bonferroni correction. Only statistical significant differences are shown. , , and .

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