FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids

Figure 3

Intake of a high fat ω3-PUFA diet alleviates hepatic lipid accumulation in Ffar4 HET and KO mice. Mice were euthanized in nonfasting state 36 weeks after diet initiation. Gene expression levels were analyzed by RT-qPCR and lipid levels were evaluated by thin-layer chromatography. ((a)–(f)) Heterozygotes. ((g)–(l)) Knockouts. ((a) and (g)) mRNA level of Scd1, –8. ((b) and (h)) mRNA level of Fas, –8. ((c) and (i)) mRNA level of Acox1 –8. ((d) and (j)) mRNA level of Mcad, –8. ((e) and (k)) mRNA level of Pparγ2, –8. ((f) and (l)) Triacylglycerol content, –8. Data are presented as means ± SEM. Both HFDs have been compared to the LFD. All data have been Ln-transformed and 1-way ANOVA with Bonferroni correction was performed. Only statistical significant differences are shown. , , and .