FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids

Figure 4

Intake of a high fat ω3-PUFA diet alleviates hepatic steatosis in Ffar4 HET and KO mice. Mice were euthanized in nonfasting state 36 weeks after diet initiation. Gene expression levels were analyzed by RT-qPCR and protein levels were evaluated by western blot analysis. ((a)–(e)) Heterozygotes. ((f)–(j)) Knockouts. ((a) and (f)) mRNA level of Il-6,  –7. ((b) and (g)) Protein level of NFκB, –8. ((c) and (h)) mRNA level of Irs2, –8. ((d) and (i)) mRNA level of Nrf1, –8. ((e) and (j)) mRNA level of Mcp-1, –8. Data are presented as means ± SEM. Both HFDs have been compared to the LFD. All data have been Ln-transformed and 1-way ANOVA with Bonferroni correction was performed. Only statistical significant differences are shown. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)