FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids

Figure 5

Inflammatory status of eWAT is indistinguishable between Ffar4 HET and KO mice. Mice were euthanized in nonfasting state 36 weeks after diet initiation. Gene expression levels were analyzed by RT-qPCR. ((a)–(e)) Heterozygotes. ((f)–(j)) Knockouts. ((a) and (f)) mRNA level of Tnfα, –8. ((b) and (g)) mRNA level of Il-6, –8. ((c) and (h)) mRNA level of Mcp-1, –8. ((d) and (i)) mRNA level of Cd68, –8. ((e) and (j)) mRNA level of Socs3, –8. Data are presented as means ± SEM. Both HFDs have been compared to the LFD. All data have been Ln-transformed and subjected to 1-way ANOVA with Bonferroni correction. , , and .