CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells

Figure 2

Knockdown of CYLD by siRNA promotes the growth of A549 and H460 cells. (a) The A549 cells were plated into 24-well plate. Eight hours later, three pairs of CYLD-siRNA were transfected into A549 cells. The transfected cells were cultured for 48 hours; the expression of CYLD was detected by western blotting analysis. (b) β-Actin was used as an internal reference and N.C. siRNA was used as the negative control. The expression level of CYLD was normalized to the expression of β-actin. , compared with the N.C. siRNA transfected group. (c) Cell viability was determined by MTT assay. The transfected A549 cells were cultured for 24 hours, 48 hours, and 72 hours, respectively, and the values were detected by MTT assay. , compared with N.C. siRNA group. (d) H460 cells were transfected with three pairs of siRNA specific to CYLD and cultured for 24 h, 48 h, and 72 h, respectively. The cell viability was determined by MTT assay. , , compared with N.C. siRNA group.

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