CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells

Figure 5

Overexpression of CYLD enhances TNF-α-induced cell necrosis of lung cancer cells. (a) The necrosis was determined by FACS assay with PI staining. The CYLD-flag plasmid was used to transfect the lung cancer cell line H460 cells. The stable transfected cell line was screened as described in Material and Method. Increasing concentration of TNF-α, including 0 ng/mL, 1 ng/mL, 20 ng/mL, and 400 ng/mL, respectively, was used to treat the CYLD-flag plasmid transfected H460 cells. (b) The PI-positive rates of CYLD-flag transfected H460 cells were shown in histogram. , compared with pcDNA3.1(+) transfected H460 cells. (c) The CYLD-flag transfected cells were pretreated with a pancaspase inhibitor, z-VAD-fmk at the concentration of 10 μM for 6 hours, and different concentrations of TNF-α were used to treat H460 to induce cell necrosis. The PI-positive rates of H460 cells were shown in histogram. , , compared with the group without z-VAD-fmk.

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