Negative Impact of Hypoxia on Tryptophan 2,3-Dioxygenase Function

Figure 3

Unaltered hTDO expression in HeLa-hTDO cells under hypoxic conditions. (a) Exemplary Western Blot protein analysis of hTDO and β-actin protein expression in HeLa-hTDO cells after 72 h of incubation under normoxia (20% O₂) or hypoxia (1% O₂). (b) Densitometric evaluation of Western Blot protein analyses: ratio of relative hTDO protein expression to β-actin protein expression as % of positive control SEM, independent experiments. Comparison of hTDO/β-actin protein ratio under normoxia or hypoxia via a two-tailed, paired t-test; n.s. = not significant.

(a)

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