Figure 5: GATA-3 modulates ANXA1 expression in Th0 conditions. CD4+ T cells were infected with GATA-3 up or GATA-3 down Lentivirus in Th0 condition and then stimulated with anti-CD3/CD28 (5.0 μg/mL) for 8 h to analyze ANXA1 expression by real-time PCR (a) and western bolt (b). Dual luciferase assays showed an increase after transfection of GATA-3 in 293T cells (c). . .