Conjugated Bilirubin Differentially Regulates CD4+ T Effector Cells and T Regulatory Cell Function through Outside-In and Inside-Out Mechanisms: The Effects of HAV Cell Surface Receptor and Intracellular Signaling

Figure 2

CD4+ TLs from HAV-seropositive patients with CB levels greater than 2 mg/dL have a lower degree of CD3 epsilon, CREB, and SYK phosphorylation. TCR-related intracellular signals were evaluated in CD4+ TLs lysates from pediatric patients and healthy donors by MAGPIX Technology. Representative Heat map (MFI) is shown in (a). The medians of the MFI and SD of phosphorylated CD3 epsilon (b), phosphorylated SYK (c), and phosphorylated CREB (d) from HAV+ patients with CB 0.3–2 mg/dL (), HAV+ patients with CB > 2 mg/dL (), and healthy (H) donors () are shown. Nonparametric Kruskal-Wallis for comparison between groups was used to calculate statistical significance. was considered statically significant. and .