Research Article

Anti-Inflammatory and Antinociceptive Activities of Anthraquinone-2-Carboxylic Acid

Figure 5

Effect of AQCA on the activation of signaling enzymes. (a and b) Total lysates were prepared from stomach tissues of mice treated with HCl/EtOH or aspirin that had been orally treated with AQCA (0, 3, and 30 mg/kg) or RT (ranitidine, 40 mg/kg). The total and phosphoforms of p38, JNK, Src, Syk, and IRAK1 were analyzed by immunoblotting analysis. (c) mRNA levels of inflammatory genes (iNOS, TNF-α, and COX-2) in LPS-treated RAW264.7 cells pretreated with SB203580 (SB), PP2, or piceatannol (Picea) were determined by real-time RT-PCR. Relative intensity was calculated using β-actin level and the DNR Bio-Imaging System ((a)(B) and (b) bottom panels). and compared with the control group.