Regulation of Autophagy-Related Protein and Cell Differentiation by High Mobility Group Box 1 Protein in Adipocytes

Figure 1

Effects of HFD on HMGB1 and LC3. After male mice were fed with HFD and ND for 16 weeks, serum was separated, and the epididymal adipose tissue was extracted. (a) The change of the body weight. (b) The epididymal adipose tissue, HFD (left) and ND (right). The black arrow pointed to epididymal adipose tissue. (c) The weight of epididymal adipose tissue. (d) HE staining of epididymal adipose tissue. (e) Levels of HMGB1 in peripheral blood were analyzed by ELISA. (f) The expression of autophagy protein LC3II and HMGB1 was analyzed by Western blot. (g-h) The protein levels were quantified through normalization with GAPDH, , , and for comparisons between ND and HFD mouse epididymal adipose tissues.

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