Regulation of Autophagy-Related Protein and Cell Differentiation by High Mobility Group Box 1 Protein in Adipocytes

Figure 2

Exogenous HMGB1 activated autophagy. 3T3-L1 preadipocytes were treated with HMGB1 protein (0.2 μg/mL) or PBS for 24 h accordingly. (a) Total protein was extracted, and LC3II, P62, and HMGB1 proteins were analyzed by western blot. (b–d) LC3II, P62, and HMGB1 protein were quantified and normalized to GAPDH, versus the control. NS: not significant.

(a)

(b)

(c)

(d)