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Mediators of Inflammation
Volume 2016 (2016), Article ID 1984703, 10 pages
Research Article

Potential of Inducible Nitric Oxide Synthase as a Therapeutic Target for Allergen-Induced Airway Hyperresponsiveness: A Critical Connection to Nitric Oxide Levels and PARP Activity

1The Stanley Scott Cancer Center, School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
2Department of Biomedical and Clinical Sciences, University of Milan, Milan, Italy
3Microbiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
4Pulmonary/Critical Care and Allergy/Immunology Section, School of Medicine, Louisiana State University, New Orleans, LA 70112, USA
5Eosinophilic Disorder Center in the Section of Pulmonary Diseases, Tulane University School of Medicine, New Orleans, LA 70112, USA

Received 5 February 2016; Revised 11 May 2016; Accepted 22 May 2016

Academic Editor: Alex Kleinjan

Copyright © 2016 Salome’ V. Ibba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: mice were challenged with aerosolized 3% OVA for 30 min three times on days 14, 16, and 18 for the acute asthma model or three times a week for three weeks (chronic asthma model). Other groups of mice were challenged intranasally with 1.25 μg/kg whole HDM extract on days 24, 25, and 26 for the acute asthma model or 3 times per week for a total of 4 weeks for the chronic asthma model. Control groups were not sensitized or challenged. Additional challenged groups of mice were administered i.p. 5 mg/kg L-N6-(1-Iminoethyl)lysine dihydrochloride (L-NIL) (Sigma-Aldrich) and/or olaparib (Selleckchem, Pittsburgh, PA) in saline 30 minutes after each challenge. Some groups of mice also received i.p. injections of 20 μg/kg of nitrite (NaNO2) (Sigma-Aldrich) as NO source 30 min after each challenge.

Figure S2: WT or iNOS-/- mice were subjected to OVA sensitization followed by the acute (A) or chronic (B) OVA challenge protocol as described for Figure 2. Penh was recorded 24 h after the last challenge in response to increasing doses of aerosolized MeCh. Results are plotted as maximal fold increase of Penh relative to baseline (0 mM MeCh) and expressed as mean ± SEM where n=5 mice per group. *, difference from HDM challenged mice; #, difference from control unchallenged mice p < 0.05. The data attained using L-NIL is included for comparison.

  1. Supplementary Material