N-Alkyl-Substituted Isatins Enhance P2X7 Receptor-Induced Interleukin-1β Release from Murine Macrophages

Figure 2

NAI or NAI-imine increase P2X7-mediated IL-1β release. LPS-primed J774 cells in physiological medium were preincubated in the presence of DMSO, or 1 μM isatin or isatin derivative (as indicated) for 30 min, and then in the absence or presence of 3 mM ATP for 20 min. The IL-1β concentration in cell-free supernatants was measured using an ELISA. Results are mean ± SD (-4); or compared with DMSO alone; ^†† or ^††† compared with DMSO and ATP.