N-Alkyl-Substituted Isatins Enhance P2X7 Receptor-Induced Interleukin-1β Release from Murine Macrophages

Figure 3

NAI or NAI-imine increase P2X7-mediated IL-1β release in a concentration-dependent manner. LPS-primed J774 cells in physiological medium were preincubated in the presence of ((a) and (b)) DMSO or (a) NAI or (b) NAI-imine (as indicated) for 30 min ((a) and (b)) and then in the absence or presence of 3 mM ATP for 20 min. The IL-1β concentration in cell-free supernatants was measured using an ELISA. (c) The percentage of maximal increase in ATP-induced IL-1β release was determined from (a) and (b). ((a) to (c)) Results are mean ± SD (); , , or compared with DMSO alone; ^† and ^††† compared with DMSO and ATP.

(a)  

(b)

(c)