The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo

Figure 1

Assessment of lentiviral vectors mediated VEGF gene knockdown. (a) Assessment of the efficiency of lentivirus transduction using fluorescence microscopy, ×400. (b) The quantitative analysis for lentiviral transduction. (c) Evaluation of VEGF mRNA expression in the MSC, MSC-GFP, and MSC-ShVEGF groups. (d) Detection of VEGF protein levels in the culture medium of the MSC, MSC-GFP, and MSC-ShVEGF groups, respectively. (e) Image for the measurement of VEGF protein expression in the MSC, MSC-GFP, and MSC-ShVEGF groups by western blotting analysis. (f) The quantitative result for western blotting analysis (green, GFP; ; versus MSC group and versus MSC-GFP group).

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