The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo

Figure 2

The effect of VEGF gene knockdown on the migration property of MSC. (a) The horizontal migration ability of the MSC with VEGF gene knockdown was evaluated by the scratch assay. The wound sites (area without cells) were observed and photographed at 0 and 12 hours (×40). (b) The bar graph showed the quantitative results of scratch healing. (c) The vertical migration ability of the MSC with VEGF gene knockdown was evaluated by Transwell migration assay. The migrating cells on the lower surface of the Transwell inserts were fixed by 4% paraformaldehyde and stained with crystal violet and observed under the microscope, ×200. (d) The bar graph showed the quantitative results of cell migration ().

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