Blockade of PLD2 Ameliorates Intestinal Mucosal Inflammation of Inflammatory Bowel Disease

Figure 3

TNF-α upregulates PLD2 expression in neutrophils. (a) Neutrophils isolated from healthy donors () were stimulated with IL-6 (10 ng/mL), IL-17A (10 ng/mL), TNF-α (10 ng/mL), IFN-γ (10 ng/mL), LPS (10 ng/mL), and IL-1β (10 ng/mL), respectively, for 4 h. Expression of PLD2 mRNA was detected by qRT-PCR. , , and versus medium alone. (b) Neutrophils isolated from healthy donors () were stimulated in vitro with different concentrations of TNF-α as indicated for 4 h; expression of PLD2 was detected by qRT-PCR. ,  , and versus that under culture in medium alone. (c) Neutrophils isolated from healthy donors () were stimulated with TNF-α (10 ng/mL) for 2, 4, and 6 h, and expression of PLD2 was detected by qRT-PCR. , , and versus that at the beginning of stimulation. Gene expression was normalized to GAPDH in each group.

(a)

(b)

(c)