Blockade of PLD2 Ameliorates Intestinal Mucosal Inflammation of Inflammatory Bowel Disease

Figure 7

Inhibition of PLD2 promotes neutrophil migration. (a) Bone marrow cells were isolated from DSS-induced mice on day 10, and expression of Ly6G and CD11b was analyzed by flow cytometry. (b) Percentages of Ly6G⁺CD11b⁺ neutrophils were calculated. and versus controls. ((c) and (d)) Neutrophils were isolated from the bone marrow of mice; expression of CXCR2 (c) and GRK2 (d) was examined by qRT-PCR. and versus controls. (e) Neutrophils were isolated from the bone marrow of mice on day 10 after DSS-induced colitis and stimulated in vitro with PLD2 inhibitor (CAY10594, 10 μm) for 30 min; neutrophil migration was then analyzed by a Transwell plate (5 μm) under the stimulation with CXCL1 (0, 50, 100, 200 ng/mL) for 30 min. and versus controls.

(a)

(b)

(c)

(d)

(e)