Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice

Figure 4

Flow cytometry of microglia and infiltrating leukocytes 3 days after SCI. (a) Gating strategy: FSC/SSC was used to define cell populations. Singlet cells were identified using FSC-A/FSC-H, and only live cells were included. (b) Representative flow cytometry plots comparing macrophage, granulocyte, and T cell infiltration in and mice. (c, d) Number (c) and percentage (d) of microglia (CD11b⁺), macrophages (CD11b⁺Gr1⁻), granulocytes (CD11b⁺Gr1⁺), and T cells (CD45⁺CD3⁺) in and mice 3 days after SCI. Results are expressed as mean ± SD,  mice/group, Mann–Whitney test.

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