Shikonin Attenuates Concanavalin A-Induced Acute Liver Injury in Mice via Inhibition of the JNK Pathway

Figure 4

Effects of shikonin on apoptosis and autophagy in mice with ConA-induced acute hepatitis. (a) cDNA levels of LC3, P62, Bcl-2, and Bax were measured with real-time PCR (, for NC versus ConA, for ConA + shikonin (7.5) versus ConA, and for ConA + shikonin (12.5) versus ConA). (b) Protein expression of LC3, Beclin-1, P62, Bcl-2, Bax, and caspase 9 was detected with western blotting and the gray values were calculated (, for NC versus ConA, for ConA + shikonin (7.5) versus ConA, and for ConA + shikonin (12.5) versus ConA). (c) Immunohistochemistry staining (×200) showed the expression of Bcl-2, Bax, and LC3 protein in liver tissue at 6 h. The ratio of brown area to total area was analyzed with Image-Pro Plus (v 6.0) (, for NC versus ConA, for ConA versus ConA + shikonin).

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