GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway

Figure 6

GEN-27 suppressed LPS-induced NF-κB p65 activation in HCT116 cells. HCT116 cells were treated with LPS (10 μg/mL) together with indicated concentrations of GEN-27 and genistein (100 μM) for the indicated times. (a–f) The nuclear translocation and protein levels of NF-κB/p65, p-IκBα, IκBα, p-IKKα/β, and IKKα/β were determined by Western blot. Data shown are representative of three experiments. The relative expressions of total proteins p-IκBα, IκBα, p-IKKα/β, and IKKα/β were normalized to β-actin. (i) The localization of NF-κB p65 was visualized using fluorescence microscopy after immunofluorescence staining with NF-κB p65 antibody (green). Cells were also stained with DAPI for visualization of the nuclei (blue). Data (means ± SDs) were representative of at least three independent experiments. and compared with control; and versus LPS alone or corresponding LPS group at indicated time.

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