SOCS1 Mimetic Peptide Suppresses Chronic Intraocular Inflammatory Disease (Uveitis)

Figure 3

SOCS1-KIR suppressed the infiltration of inflammatory cells into the retina during EAU. We induced EAU in rats by adoptive transfer of uveitogenic R16 T cell line (a, c, d) or by active immunization with R16 peptide (b) and the rats were treated with SOCS1-KIR or scrambled peptide (control). Inflammatory cells present in the retina on day 12 after disease induction were analyzed by flow cytometry. Numbers in quadrants indicate percentage of T lymphocytes (CD4⁺, CD8⁺) or myeloid cells (CD11b/c⁺) in the retinae. (c, d) Ocular aqueous and vitreous humor of rats were collected 50 days after adoptive transfer. We smeared 1 mL ocular fluid from each rat eye on the glass slide and stained for cytological analysis with giemsa stain (c) and the cells were then quantified (d). Results represent at least three independent experiments. < 0.05, < 0.005, and < 0.001.

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