SOCS1 Mimetic Peptide Suppresses Chronic Intraocular Inflammatory Disease (Uveitis)

Figure 5

SOCS1-KIR induced expansion of IL-10-producing T cells while suppressing Th17 cells. We isolated cells from the spleen and LN of rats with EAU, restimulated the cells in vitro with the R16 peptide in medium containing SOCS1-KIR or scrambled peptide, and then analyzed the cells by the thymidine incorporation assay (a), intracellular cytokine staining assay (b, c), or qPCR (d). Proliferative responses were analyzed in six replicate cultures and data presented as CPM indicate the mean values of six replicate cultures. For intracellular staining, the cells were gated on CD3⁺ T cells and analysis was on CD4⁺ T cells. Numbers in quadrants (b, c) indicate percentage of IL-10-, IL-17-, or IFN-γ-expressing CD4⁺ T cells. Results represent three independent experiments. < 0.05, < 0.005, < 0.001 (Student’s two-tailed -test).

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