Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation

Figure 1

IL-17 enhances MMP-9 production in C2C12 myoblast cells. (a) C2C12 cells were treated with increasing concentrations of IL-17 for 24 h. MMP-9 activity in conditioned media was determined by zymography and MMP-9 expression was determined by RT-PCR analysis. GAPDH was used as a gel loading control. MMP-9 activity is observed in form of clear bands in the gel. Quantification plot is presented above the zymography. (b) MMP-9 promoter transactivity in C2C12 cells transiently transfected with mouse pMMP-9-luc reporter plasmid and treated with increasing amounts of IL-17 for 24 h. Significant difference from the control (cells not treated with IL-17) by -test: and .

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