Research Article
Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation
Figure 3
Doxycycline reverts myogenesis inhibition induced by IL-17. (a) Hypertrophy index of myotubes was measured after 4 days of cultivation in differentiation medium with or without Doxy and IL-17. Hypertrophic myotubes are determined by the average myotube diameter. (b) MyHC protein expression under conditions described above determined by Western blot. α-tubulin is used as gel loading control. (c) MCK expression under conditions described above determined by RT-PCR. GAPDH is used as gel loading control. (d) Myogenin promoter (pG133-luc) transactivity determined by luciferase assay. RLU: relative luciferase activity.
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