Correlation of Surface Toll-Like Receptor 9 Expression with IL-17 Production in Neutrophils during Septic Peritonitis in Mice Induced by E. coli

Figure 4

The ratios of sTLR9⁺ PMNs and macrophages in PLCs from mice infected intraperitoneally with E. coli. ICR mice were infected intraperitoneally with 3 doses of E. coli or received saline and sacrificed at 18, 48, and 72 hours to harvest their PLCs. The PLCs were stained with fluorescence-labeled mAb of anti-CD45, anti-CD11b, and anti-TLR9 followed by detection with flow cytometry. (a) Gates of the PMNs from the CD45⁺ PLCs followed by gating CD11b⁺ PMNs or CD11b⁻ PMNs from the PMNs. (b) Ratios of sTLR9⁺ PMNs in CD11b⁺ PMNs. (c) Ratios of sTLR9⁺ PMNs in CD11b⁻ PMNs. (d) Ratios of sTLR9⁺ macrophages. Each point represents the data from one mouse.

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