Correlation of Surface Toll-Like Receptor 9 Expression with IL-17 Production in Neutrophils during Septic Peritonitis in Mice Induced by E. coli

Figure 5

The ratios of IL-17⁺ PMNs and macrophages in PLCs from mice infected with E. coli intraperitoneally. ICR mice were infected with E. coli at 3 doses or received saline and sacrificed at 18, 48, and 72 hours to harvest their PLCs. The PLCs were stained with fluorescence-labeled mAb of anti-CD45, anti-CD11b, and anti-IL-17, followed by detection with flow cytometry or lysed to isolate total RNA for amplifying IL-17 mRNA by qPCR. (a) PMNs gated from the CD45⁺ PLCs (gate 1) and CD11b⁺ PMNs or CD11b⁻ PMNs from the gated PMNs (gate 2). (b) Ratios of IL-17⁺ PMNs in CD11b⁺ PMNs. (c) Ratios of IL-17⁺ PMNs in CD11b⁻ PMNs. (d) Ratios of IL-17⁺ macrophages. (e) Percentages of IL17⁺ PLCs in the PLCs cocultured with E. coli. (f) Copies of IL-17 mRNA in the PLCs. Each point represents the data from one mouse. (g) IL-17 mRNA expression in sorted CD3⁺ cells or CD3⁻CD14⁻CD11b⁺ neutrophils.

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