Correlation of Surface Toll-Like Receptor 9 Expression with IL-17 Production in Neutrophils during Septic Peritonitis in Mice Induced by E. coli

Figure 6

The expression of IL-17 and sTLR9 in/on PMNs, macrophages, and T cells in the PLCs from mice infected with E. coli intraperitoneally at early stage after infection. ICR mice which were infected with E. coli at 3 doses or received saline were sacrificed at 8 hours after infection for harvesting their PLCs. The PLCs were stained with fluorescence-labeled mAb of anti-CD45, anti-CD11b, anti-IL-17, anti-sTLR9, anti-CD3, and anti-Ly6G, followed by detection with flow cytometry. The expression levels of IL-17 and sTLR9 in various cells of the PLCs were indicated by mean fluorescence intensity (MFI). (a) IL-17 in CD11b⁻ PMNs. (b) IL-17 in CD11b⁺ PMNs. (c) IL-17 in macrophages. (d) sTLR9 on CD11b⁻ PMNs. (e) sTLR9 on CD11b⁺ PMNs. (f) sTLR9 on macrophages. (g) sTLR9 on CD11b⁺Ly6G⁺ PMNs. (h) IL-17 in CD11b⁺Ly6G⁺ PMNs. (i) IL-17 in sTLR9⁺CD11b⁺Ly6G⁺ PMNs. (j) IL-17 in CD3⁺ PLCs. (k) IL-17 in CD3⁻CD11b⁺ PMNs. Each point represents the data from one mouse.

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