Regulatory T Cell Induced by Poria cocos Bark Exert Therapeutic Effects in Murine Models of Atopic Dermatitis and Food Allergy

Figure 5

Immunomodulatory effects of PCB extract on cytokine expression patterns in mesenteric lymph nodes of food allergic mouse. Mice were sacrificed by cervical dislocation on day 35, and mLNs were isolated. mLNs were cultured in RPMI medium containing 10% fetal bovine serum for 72 h. Cytokines secreted from mLNs were quantified by ELISA. Interleukin- (IL-) 4 (a), IL-5 (b), IL-13 (c), and TGF-β (d) were measured. (e) The expression of Foxp3 in gated CD4⁺ cells was analyzed using flow cytometry. Data are presented as the mean of the percentage of Foxp3⁺CD4⁺ T cells from three independent experiments and as mean ± SD. Data were analyzed using one-way repeated measure ANOVA followed by Dunnett’s post hoc test. Bars represent significant differences from the sham group at , , and .

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