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Figure 4: Characterization of IL-4-producing cells in the LC. (a–c) Representative fluorescent images showed that IL-4-secreting cells (a) were colocalized (c) with TH-releasing cells (b). Photomicrographs were taken at ×200 magnification. Scale bars = 50 μm. (d–f) High power (taken at ×400 magnification) photographs of (a), (b), and (c), respectively, are shown. Scale bars = 20 μm. IL-4-releasing cells (g and j) were not merged (i and l) with GFAP-positive cells (h) or Iba-1-immunoreactive cells (k). Photomicrographs were taken at ×400 magnification. Scale bars = 20 μm.