In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

Figure 5

Effects of pretreatment with 10-H2DA, 10-HDAA, and SEA on the mRNA expression of key inflammatory-mediators and cytokine genes in LPS-stimulated RAW 264.7 cells. Effects of 10-H2DA, 10-HDAA, and SEA on the mRNA expression of key inflammatory-mediators and cytokine genes in LPS-stimulated RAW 264.7 cells. Cells were pretreated with 10-H2DA, 10-HDAA, and SEA in designed concentrations for 1 h and then stimulated with LPS (1 μg/mL) for 30 min. The mRNA levels of IL-6 (a), IL-10 (b), iNOS (c), COX-2 (d), TNF-α (e), and HO-1 (f) were quantified using qRT-PCR and normalized to GAPDH and the levels of gene expression in the LPS group were set to 1. Data shown represent means ± SEM values from three independent experiments. One-way ANOVA with the Student–Newman–Keuls method was performed to compare all groups; means with different letters are significantly different ().

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