In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

Figure 6

Effects of 10-H2DA, 10-HDAA, and SEA on the phosphorylation of MAPK and NF-κB pathways in LPS-stimulated RAW 264.7 cells. (a) RAW 264.7 cells either were pretreated with 10-H2DA, 10-HDAA, and SEA at the indicated concentrations for 1 h or received no such pretreatment. They were then stimulated with LPS (1 μg/mL) for 30 min. Whole cell lysates were analyzed by Western blotting analysis using specific antibodies. The relative expression of proteins was quantified using Quantity One software, comparing with β-tubulin. Data shown are the representative of three independent experiments with similar results. (b–g) The intensity of corresponding bands was measured by densitometry and normalized to β-tubulin. The values are the means ± SEMs. Individual groups were compared by Student’s t-test (, , and compared with the LPS group; compared with untreated group).

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