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Mediators of Inflammation
Volume 2016, Article ID 3630485, 12 pages
http://dx.doi.org/10.1155/2016/3630485
Research Article

Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice

1Department of Nursing, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan
2Chang Gung Memorial Hospital at Linkou, Guishan District, Taoyuan City 33303, Taiwan
3Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan
4Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan
5Graduate Institute of Health Industry Technology, Chang Gung University of Science Taiwan and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan

Received 8 September 2015; Revised 8 November 2015; Accepted 10 November 2015

Academic Editor: Mirella Giovarelli

Copyright © 2016 Chian-Jiun Liou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Matrine is isolated from Sophora flavescens and shows anti-inflammatory effects in macrophages. Here we evaluated matrine’s suppressive effects on cyclooxygenase 2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expressions in lipopolysaccharide- (LPS-) stimulated human lung epithelial A549 cells. Additionally, BALB/c mice were given various matrine doses by intraperitoneal injection, and then lung injury was induced via intratracheal instillation of LPS. In LPS-stimulated A549 cells, matrine inhibited the productions of interleukin-8 (IL-8), monocyte chemotactic protein-1, and IL-6 and decreased COX-2 expression. Matrine treatment also decreased ICAM-1 protein expression and suppressed the adhesion of neutrophil-like cells to inflammatory A549 cells. In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. In vivo data indicated that matrine significantly inhibited neutrophil infiltration and suppressed productions of tumor necrosis factor-α and IL-6 in mouse bronchoalveolar lavage fluid and serum. Analysis of lung tissue showed that matrine decreased the gene expression of proinflammatory cytokines, chemokines, COX-2, and ICAM-1. Our findings suggest that matrine improved lung injury in mice and decreased the inflammatory response in human lung epithelial cells.