Osteoclasts Are Required for Hematopoietic Stem and Progenitor Cell Mobilization but Not for Stress Erythropoiesis in Plasmodium chabaudi adami Murine Malaria

Figure 4

Impact of calcitonin on bone marrow hematopoiesis during Plasmodium infection. Mice were treated with calcitonin (white bars; Cal) or with PBS (grey bars; Ctl) during 5 consecutive days and were infected with P. c. adami DK parasites (10⁵ pRBCs) on the 3rd day of treatment. Data from uninfected controls (Ctl, Cal ) is included for comparison. Mice were sacrificed 5 (), 8 (–12), or 10 (-4) days after infection, and femoral and tibia bone marrow was aseptically recovered for analysis of numbers of total cells (a), erythroid cells (b), lineage⁻ cells (c), and hematopoietic stem cells (d) by cytofluorometry. Data are mean ± SEM from 3 independent experiments (a total of 19–24 mice per infected group); values are compared to respective uninfected control mice and between the two infected groups using a one-way ANOVA test. ; ; .

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