New Role of Adult Lung c-kit⁺ Cells in a Mouse Model of Airway Hyperresponsiveness

Figure 2

In vivo engraftment of GFP-labeled c-kit⁺ cells and pulmonary function. (a) Scheme of in vivo experiments. (b) Efficiency of GFP transfection. Phase contrast and GFP fluorescence image of living c-kit⁺ cells after lentiviral transduction. (c) Airway reactivity to acetylcholine (ACh, in moles) as change in resistance (RL) in control, asthmatic (OVA), c-kit⁺ cell-treated (OVA+cCs), and fibroblast-treated (OVA+Fibroblasts) mice. (d) Detection of GFP gene by PCR in the lungs of vehicle- and c-kit⁺ cell-treated mice. (e, f) Engraftment of GFP-labeled cells after intratracheal administration. Scale bars, (e, f) 20 μm. versus control; versus OVA; versus OVA+Fibroblasts.

(a)

(b)

(c)

(d)

(e)

(f)