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Mediators of Inflammation
Volume 2016, Article ID 4158287, 13 pages
http://dx.doi.org/10.1155/2016/4158287
Research Article

Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion

1Department of Gynecology, Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, China
2Department of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan, China
3Department of OB/GYN, Baylor College of Medicine, 6651 Main Street, 10th Floor, Houston, TX 77030, USA
4Center for Medical Experiments, Third Xiangya Hospital, Central South University, Changsha, Hunan, China

Received 8 November 2015; Revised 19 January 2016; Accepted 26 January 2016

Academic Editor: Marc Pouliot

Copyright © 2016 Umme Salma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was to evaluate the role of Smad3, Smad7, and TGF-β1 in intrauterine adhesion (IUA) patients and experimental rabbit models. 60 IUA patients, 30 control participants, and 18 female rabbits were enrolled in this study. We found that the plasma concentrations and protein expressions of TGF-β1 were significantly increased in patients and experimental rabbits compared to those in controls (). Furthermore, the mRNA and protein expression levels of Smad3 were significantly elevated, while Smad7 level was markedly decreased in the patients and experimental rabbits compared with controls (). This altered ratio recommended that IUA was positively correlated to the mRNA and protein expression levels of Smad3, Smad7, and TGF-β1 in blood and uterine tissue. Moreover, we used the specific inhibitor of Smad3 (SIS3) in experimental rabbit. SIS3 obviously reduced the mRNA and protein expression of smad3 and TGF-β1, while it increased Smad7 expression in the treatment groups as compared with IUA rabbits (). Our study suggested that TGF-β1/Smad3/smad7 is a major pathway which plays an important role in the regulation of the IUA and specific inhibitor of Smad3 (SIS3) may provide a new therapeutic strategy for IUA.