Research Article

Kinin Peptides Enhance Inflammatory and Oxidative Responses Promoting Apoptosis in a Parkinson’s Disease Cellular Model

Figure 5

The effect of MPP+ on D2R expression in differentiated SK-N-SH cells. Confluent cells were incubated with MPP+ at different time intervals. D2R mRNA expression was analyzed by Real-Time PCR (a). The figure represents changes of mRNA expression after different times of incubation in comparison to untreated cells assumed to possess value equal to 1 (dashed line). The expression of D2R protein was analyzed by immunocytochemistry with specific antibodies and the quantification of the fluorescence signal was performed as described in Materials and Methods (b). The results are shown as mean ± SD of the changes in comparison to untreated cells, assumed to show 100% expression (dashed line). (c) The photographs show D2R expression by differentiated SK-N-SH cells without or after 5-hour incubation with MPP+. versus untreated cells.