Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats

Figure 5

TLR4 inhibition significantly attenuated apoptosis after DAI. (a) TUNEL assay was used to detect apoptotic cells in the cortices of rats in the control, DAI 1 d, DAI 1 d+vehicle, and DAI 1 d+TAK-242 groups. TUNEL-positive cells were stained green, and the nuclei were stained with DAPI (blue). Scale bar = 100 μm; enlarged figure scale bar = 20 μm. (b) The bar graphs show the results regarding the numbers of apoptosis cells, which are expressed as the ratio of TUNEL to total nuclei. Positive cell levels were counted in 5 random cortical fields (40x magnification). Data are presented as the mean ± SD (; , compared with control group; , , compared with DAI 1 d+vehicle group).

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