Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats

Figure 7

TLR4 inhibition attenuated neuronal and axonal injury. (a) Microtubule-associated protein tau immunoreactivity and neuronal nuclei protein (NeuN) immunoreactivity were assessed via immunohistochemistry in rat cortices in the control, DAI 1 d, DAI 1 d+vehicle, and DAI 1 d+TAK-242 groups. Scale bar = 50 μm; enlarged figures scale bar = 20 μm. (b) The bar graphs show the results for tau and NeuN immunoreactivity, as determined via immunohistochemistry. Tau and NeuN immunoreactivity was assessed in 5 random fields (40x magnification) via immunohistochemical scores (IHS). (c) The expression of tau and NeuN was measured via western blotting. (d) The bar graphs show the results for tau and NeuN expression, as assessed via western blotting. Data are presented as the mean ± SD (; , compared with control group; , , compared with DAI 1 d+vehicle group).

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