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Mediators of Inflammation
Volume 2016 (2016), Article ID 4851935, 11 pages
Research Article

Effects of Acute Endurance Exercise on Plasma Protein Profiles of Endurance-Trained and Untrained Individuals over Time

1Department of Sports Medicine, Justus-Liebig University Giessen, Kugelberg 62, 35394 Giessen, Germany
2Mathematical Institute, Justus-Liebig University Giessen, Arndtstraße 2, 35392 Giessen, Germany
3Department of Sports Medicine, University Hospital of Tuebingen, Hoppe-Seyler-Straße 6, 72076 Tuebingen, Germany
4Division of Sport and Rehabilitation Medicine, University Hospital Ulm, Parkstraße 11, 89075 Ulm, Germany

Received 12 January 2016; Revised 7 March 2016; Accepted 28 March 2016

Academic Editor: Julio Galvez

Copyright © 2016 Marius Schild et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Acute physical exercise and repeated exercise stimuli affect whole-body metabolic and immunologic homeostasis. The aim of this study was to determine plasma protein profiles of trained (EET, ) and untrained (SED, ) individuals at rest and in response to an acute bout of endurance exercise. Participants completed a bicycle exercise test at an intensity corresponding to 80% of their . Plasma samples were taken before, directly after, and three hours after exercise and analyzed using multiplex immunoassays. Seventy-eight plasma variables were included in the final analysis. Twenty-nine variables displayed significant acute exercise effects in both groups. Seven proteins differed between groups, without being affected by acute exercise. Among these A2Macro and IL-5 were higher in EET individuals while leptin showed elevated levels in SED individuals. Fifteen variables revealed group and time differences with elevated levels for IL-3, IL-7, IL-10, and TNFR2 in EET individuals. An interaction effect could be observed for nine variables including IL-6, MMP-2, MMP-3, and muscle damage markers. The proteins that differ between groups indicate a long-term exercise effect on plasma protein concentrations. These findings might be of importance in the development of exercise-based strategies in the prevention and therapy of chronic metabolic and inflammatory diseases and for training monitoring.