Research Article

Cannabinoid CB2 Receptor Mediates Nicotine-Induced Anti-Inflammation in N9 Microglial Cells Exposed to β Amyloid via Protein Kinase C

Figure 2

Nicotine increased CB2 receptor expression in microglial cells exposed to Aβ. The microglial cells were divided into five groups: control: cells cultured in drug-free medium; Aβ: cells cultured in the medium containing 5 μM Aβ; nicotine (Nico) + Aβ: cells cultured in the medium containing 10 μM nicotine and 5 μM Aβ; AM630 + Nico + Aβ: cells cultured in the medium containing 10 μM CB2 antagonist AM630, 10 μM nicotine, and 5 μM Aβ; AM630 + Aβ: cells cultured in the medium containing 10 μM AM630 and 5 μM Aβ. After an incubation of 24 h, immunocytochemistry was used in investigating CB2 receptor expression (red), and the nuclei (blue) were counter-stained with DAPI staining solution. Bar = 20 μm.