An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons

Figure 2

Cyclic AMP/PKA pathway facilitates NMDA-induced outward current in the acutely dissociated mouse cortical neurons. (a, b) Intracellular perfusion of cAMP (10 μM) facilitated NMDA-induced outward currents (a), whereas KT5720 (10 μM), a specific inhibitor of PKA, abolished them (b). Upper trace is continuous recording of NMDA-induced currents. Lower traces are enlarged traces of upper trace. An arrow indicates the prolonged NMDA-induced outward current. Calibration bars are 50 pA and 20 sec applicable to lower traces. (c) Time course of the potentiation of NMDA-induced outward currents by intracellular perfusion of cAMP. The circles and bars represent the mean ± SEM ( cells). Asterisks indicate a significant difference from the none-treated control (, one-way ANOVA with post hoc Dunnett’s test). Swords indicate a significant difference between cAMP and cAMP+KT5720 (, = 39.32, two-way ANOVA Bonferroni test).

(a)

(b)

(c)