Folic Acid Is Able to Polarize the Inflammatory Response in LPS Activated Microglia by Regulating Multiple Signaling Pathways

Figure 1

(a) Effects of folic acid on LPS-induced NO production in BV-2 cells. Cells were preincubated in medium containing various concentrations of folic acid (FA) (5, 10, 30, and 50 μg/mL) for 1 h and then treated with LPS (1 μg/mL) for 24 h. The amount of nitrite in the medium was measured by Griess reaction. (b) iNOS expression in BV-2 cells. Total cellular protein samples were collected and examined by western blotting. Protein levels were determined by measuring immunoblot band intensities by scanning densitometry. (c) iNOS mRNA expression determined by real-time RT-PCR. BV-2 microglial cells were incubated for 6 h with LPS alone, or after 1 h of pretreatment with different amounts of folates. Experimental treatments were analyzed in triplicate and data are expressed as the mean ± SD of five independent experiments. .

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